Actual location is: Compound Submission /
IP Protection
Structural data of compounds spended by chemists of the network are only provided with the permission of the spender. Projects which identify hits in spended libraries have to contact the chemist who created the molecules to request structural informations. Publication or patent protection of results is negotiated between chemists and screening projects based on individual collaborations between the partners and their institutions.
ChemBioNet Standards
According to the conventions of the ChemBioNet as defined by the partnering institutions, the compounds should have a purity of 90%.
The compounds must be delivered with structural information in the sdf fileformat to avoid errors in actualization of the database. The file should contain an identifyer-code referring to the internal data of the provider. Additional information according to restrictions of usage or contribution of third parties must me provided.
The compounds may be sent as dry powder or already prepared on microtiterplates in 10 mM solutions in DMSO.
Plate formats are defined by columns without compounds but DMSO for column 12 on 96well and columns 23 and 24 on 384well plates. 384well plates are combined from 96 well plates according to the z-scheme (plate1:A1; plate2:A2;plate3:B1; plate4:B2).
We provide REMP boxes (96 tube format, 200 µl volumes, column 12 empty) for compound submission on request!
See also: "Screening@FMP" and "Compound Management" within this context.
Compounds can be delivered in the following formats:
As powder: 0,5-5mg are sufficient
As solution: 10mM in DMSO
96-well plate: columns 1-11, column 12 empty, 80-180 µl/well
384-well plate: columns 1-22, column 23 & 24 empty, 30-45µl /wellFor information on storage plates see appendix A.
Handling compounds delivered in powder form:
Weigh out compounds into 2ml tubes without gloves
Dissolve compounds to a final concentration of 10mM
using DMSO p.a. (e.g. Roth Art. Nr. 4720.3)
Shake tubes for 10-30 min
Spin down tubes to avoid contaminations at the lid
Transfer compounds into 96 well plate, Column 1- 11, 80-180µl/well
Seal plates with heat-seal or adhesive lids, see appendix.
B. Handling large scale libraries
These libraries are typically delivered in powder form in 96-well plates. If compounds are delivered in fixed milligram amounts calculate roughly volume required to prepare 10mM solution. Final molarity will depend on the variations in the MW of the different compounds.
Dissolve compounds and assemble 384-well plate.
C. How to assemble a 384-well plate
Four 96-well plates are assembled into one 384-well plate according to the following z-scheme:
1st 96-plate: upper-left quadrant
2nd 96-plate: upper-right quadrant
3rd 96-plate: lower-left quadrant
4th 96-pate: lower-right quadrantEach
384-well plate should contain about 30-45µl / well
Make 5 copies if possible:
A: Backup, B: Screening I, C: Screening II, D: Screening III,
E: cherry-picking
Keep 96-well plate as back-up
Seal plates with lids, see Appendix
Rub lid firmly on the plates until you can clearly see the pattern of the plate Store plates at –20°C up to –80°C