Compounds are stored in racks containing 96 separate tubes (volume 200 µl each). For reformatting selected tubes are punched into empty racks within the freezer in an order defined by worksheets. Caps are removed to allow compound transfer by a 96-tip pipetting-head into 384well plates according to the z-scheme defined by the ChemBioNet.
Replicates in 384well format are sealed with Aluminium foil by a heat sealing automat.
Each tube of the mother plates (96-tube racks) is marked by a barcode at bottom, which is scanned on the deck by a barcode reader for control. Thawing of mother plates is accellerated by an incubator (blue).
Automated Library Management (REMP, Tecan AG)
The REMP-system in combination with a pipetting automat enables automated library reformating and hitpicking, already within the compound storage freezer at -20° C. The system protects compounds from degradation through repeated freeze-thaw cycles, because only required compounds stored in separate vials are punched into new boxes directly in the freezer and are thawn on the deck of the pipetting robot for generation of replicates, sublibraries or hitpicking plates for further usage. Tracking of compound usage within an Oracle database supports compound management. New compound solutions are transfered to REMP-vials by a flexible 8-channel pipetting tool (LiHa) on the deck of the liquid handling system. Individual probes or complete 96-tube racks are imported into the storage system via the FreedomEvoware System.
The Small Size Storage System has a capacity of about 100.000 compounds.
